摘要:从被油污染的土壤中获取菌源,对混合菌种进行富集驯化,研究了富集驯化过程中油的降解率变化。通过正交试验研究了混合茵液的降解能力。结果发现,第一天油的降解率达到77%,随后逐渐减慢。最佳工艺参数为:温度40℃,pH 7.5~8.5,接种量60%,Na2Si03.9H20 5~7 g/L,Na3P04-12H20 5—20 g/L,OP一10 2 g/L,摇床转速150 r/min。除油试验表明,在电镀前处理中利用微生物除油是可行的。 关键词:生物除油剂;微生物;电镀;前处理;正交试验 中图分类号:TGl78 文献标志码:A 文章编号:1004—227X(2009)03—0034—04 Preliminarily study on biological degreasing agent//WANG Guang—yu,CHEN Lei,MA Mei—rong*,LI Ning,MA Fang,DIAO Hong-cheng Abstract:A bacteria source was obtained from oil-polluted soil.Mixed bacterial species were enriched and domesticated while the oil degradation rate was studied.The degradation capability of the mixed bacterial species was investigated by orthogonal test.The results showed that the degradation rate is up t0 77%in the first day,then slowly decreased.The optimal process parameters were determined as follows:temperature40 ℃,pH 7.5—8.5,inoculation dose 60%,Na2Si03·9H20 5-7 g/L,Na3P04·12H20 5-20 g/L,OP-102 g/L,and speed of mtary shaker l 50 r/min.The degreasing test proved that oil removal using microorganisms is feasible for the application to electroplating pretreatment. Keywords:biological degreasing agent;microorganism;electroplating;pretreatment;orthogonal test First-author’S address:Ocean College,Harbin Institutes of Technology at Weihai,Weihai 264209,China
1 前言
金属件电镀及涂装前的除油处理是保证镀层及涂层质量的重要手段。目前,除油处理采用的方法主要有喷砂法、燃烧法、溶剂法、电化学法、化学清洗法及其他多功能水剂除油法。其中,化学清洗投资少、效果好、操作容易、管理方便,是目前应用最多的一种除油处理方法。传统化学除油存在的缺点是使用时必须加热,常温下除油效果差,除油效果随着槽液的老化而逐渐变弱,既浪费资源又造成油污、泥渣的二次污染,还存在高泡沫性和高碱性等问题。因此,寻找一种低成本、无污染、安全性好的室温除油剂,对节约能源、降低能耗、保护环境具有深远的意义[l]。本文对除油微生物进行了富集驯化,研究了钢铁表面微生物除油的影响因素,以期获得可在室温条件下使用的低成本、无污染的高效微生物除油剂。
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